DyLight™ 554偶联标记鬼笔环肽素，细胞骨架标记荧光染料,300unit DyLight™ 554 Phalloidin,300 units

DyLight™350偶联标记的鬼笔环肽，细胞骨架标记荧光染料,300unit DyLight™350 Phalloidin, 300units

DyLight™488偶联标记的鬼笔环肽，细胞骨架标记荧光染料,300unit DyLight™488 Phalloidin, 300units

DyLight™549偶联标记的鬼笔环肽，细胞骨架标记荧光染料,300unit DyLight™594 Phalloidin, 300units

DyLight™650偶联标记的鬼笔环肽，细胞骨架标记荧光染料,300unit DyLight™650 Phalloidin, 300units

Alexa Fluor® 647标记的鬼笔环肽，细胞骨架标记荧光染料。一种自然出现的有毒的二环肽，存在于剧毒毒伞的子实体菌、鬼笔鹅膏中，对丝状肌动蛋白有很强的亲和力。对于6.6 μM (20X)浓度的储存液，将冻干粉溶于1.5 ml纯甲醇中。固定细胞，用新鲜无甲醇的4%甲醛固定15分钟，然后用PBS清洗3次，每次5分钟。一旦固定，根据CST公司提供的方案进行免疫染色。加入一抗和二抗进行孵育，Alexa Fluor® 647鬼笔环肽用PBS 1：20稀释（100μl体积中加入5μl）后加入细胞中。在室温下孵育15分钟后，用PBS清洗一次。盖玻片上滴加ProLong® Gold 抗淬灭试剂，用合适的激发波长检测样品。 300 assays. To make a stock concentration of 6.6 μM (20X), reconstitute the lyophilized material in 1.5 ml pure methanol. 
 Fix cells for 15 minutes using fresh, methanol-free 4% formaldehyde, then rinse three times in PBS for 5 minutes each. Once fixed, please follow CST protocol for immunostaining. Following incubation of the primary and secondary antibodies, Alexa Fluor® 647 Phalloidin can be diluted 1:20 (5 μl per 100 μl assay volume) in PBS and added to cells. Allow to incubate for 15 minutes at room temperature, then rinse once with PBS. Coverslip slides with ProLong® Gold antifade reagent and examine specimen using appropriate excitation wavelength. Phalloidin is a natually occuring toxic bicyclic peptide found in the deathcap toadstool, Amanita phalloides, that rapidly binds to F-actin with strong affinity. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin .

Alexa Fluor® 555标记的鬼笔环肽，细胞骨架标记荧光染料。一种自然出现的有毒的二环肽，存在于剧毒毒伞的子实体菌、鬼笔鹅膏中，对丝状肌动蛋白有很强的亲和力。对于6.6 μM (20X)浓度的储存液，将冻干粉溶于1.5 ml纯甲醇中。固定细胞，用新鲜无甲醇的4%甲醛固定15分钟，然后用PBS清洗3次，每次5分钟。一旦固定，根据CST公司提供的方案进行免疫染色。加入一抗和二抗进行孵育，Alexa Fluor® 555鬼笔环肽用PBS 1：20稀释（100μl体积中加入5μl）后加入细胞中。在室温下孵育15分钟后，用PBS清洗一次。盖玻片上滴加ProLong® Gold 抗淬灭试剂，用合适的激发波长检测样品。 300 assays. To make a stock concentration of 6.6 μM (20X), reconstitute the lyophilized material in 1.5 ml pure methanol. 
 Fix cells for 15 minutes using fresh, methanol-free 4% formaldehyde, then rinse three times in PBS for 5 minutes each. Once fixed, please follow CST protocol for immunostaining. Following incubation of the primary and secondary antibodies, Alexa Fluor® 555 Phalloidin can be diluted 1:20 (5 μl per 100 μl assay volume) in PBS and added to cells. Allow to incubate for 15 minutes at room temperature, then rinse once with PBS. Coverslip slides with ProLong® Gold antifade reagent and examine specimen using appropriate excitation wavelength. Phalloidin is a natually occuring toxic bicyclic peptide found in the deathcap toadstool, Amanita phalloides, that rapidly binds to F-actin with strong affinity. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin .

Alexa Fluor® 488偶联标记的鬼笔环肽，细胞骨架标记荧光染料，300unit，Excitation: 495, Emission: 518。 对于6.6 μM (20X)浓度的储存液，将冻干粉溶于1.5 ml纯甲醇中。固定细胞，用新鲜无甲醇的4%甲醛固定15分钟，然后用PBS清洗3次，每次5分钟。一旦固定，根据CST公司提供的方案进行免疫染色。加入一抗和二抗进行孵育，Alexa Fluor® 488鬼笔环肽用PBS 1：20稀释（100μl体积中加入5μl）后加入细胞中。在室温下孵育15分钟后，用PBS清洗一次。盖玻片随着ProLong® Gold Antifade Reagent #9071轻轻滑动，用合适的激发波长检测样品。 Alexa Fluor® 488 Phalloidin, 300assays. To make a stock concentration of 6.6 μM (20X), reconstitute the lyophilized material in 1.5 ml pure methanol. 
 Fix cells for 15 minutes using fresh, methanol-free 4% formaldehyde, then rinse three times in PBS for 5 minutes each. Once fixed, please follow CST protocol for immunostaining. Following incubation of the primary and secondary antibodies, Alexa Fluor® 488 Phalloidin can be diluted 1:20 (5 μl per 100 μl assay volume) in PBS and added to the cells. Allow to incubate for 15 minutes at room temperature, then rinse once with PBS. Coverslip slides with ProLong® Gold Antifade Reagent #9071 and examine specimen using appropriate excitation wavelength.