新一代离子交换柱，超快获得高产量无内毒素级别超纯质粒。可纯化高分子量质粒。Column filter同步完成上清过滤和质粒吸附。只需100 min，产量翻倍,250ug以上。• Plasmid DNA with less than 0.05 EU/μg for transfection of highly sensitive cells• Patented endotoxin removal by additional washing step – no extra ice incubation necessary• Optimized column design: Midi prep in approximately 45 min, Maxi prep in 50 min• Column filter included: high filter flow-rates, parallel lysate clearing and loading onto the column• Improved silica material: typical yields of 250 μg (Midi) and 1000 μg (Maxi)Technology Anion-exchange chromatography Format Midi gravity flow columnsLysate clarification Column filtersSample material Vector size Typical yield > 250 µgA260/A280 1.8–1.95Endotoxin level Isopropanol precipitation：CentrifugationPreparation time 100 min/4 prepsNucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A

新一代离子交换柱，超快获得高产量无内毒素级别超纯质粒。Column filter同步完成上清过滤和质粒吸附。NucleoBond® Finalizers滤器快速浓缩沉淀质粒，无需离心。只需45 min，产量翻倍,250ug以上。• Plasmid DNA with less than 0.05 EU/μg for transfection of highly sensitive cells• Patented endotoxin removal by additional washing step – no extra ice incubation necessary• Optimized column design: Midi prep in approximately 45 min, Maxi prep in 50 min• Column filter included: high filter flow-rates, parallel lysate clearing and loading onto the column• Improved silica material: typical yields of 250 μg (Midi) and 1000 μg (Maxi)• NucleoBond® Xtra Plus EF kits contain NucleoBond® Finalizers to speed up DNA precipitationTechnology Anion-exchange chromatography Format Midi gravity flow columnsLysate clarification Column filtersSample material Typical yield > 250 µgA260/A280 1.8–1.95Endotoxin level Isopropanol precipitation：NucleoBond® FinalizersPreparation time： 45 min/4 prepsNucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizer, buffers, RNase A 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, NucleoBond Finalizers, syringes, buffers, RNase A

经典离子交换柱，用于纯化无内毒素级别纯度的质粒DNA。可纯化高分子量质粒。载量500µg• Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells• Patented endotoxin removal by additional washing step – no extra ice incubation necessary• Fast and easy clarification of lysates by NucleoBond® Folded Filters, no centrifugation required, no shearing forces• NucleoBond® Finalizers available as separate tool to avoid time consuming centrifugation for plasmid precipitation• Transfection-grade plasmid DNA due to patented anion-exchange technology• Separate binding columns of NucleoBond® PC kits available as NucleoBond® AX columnsTechnology Anion-exchange chromatography Gravity-flow columnsFormat MaxiLysate clarification: Folded filtersSample material 30–150 mLVector size Typical yield 450–520 µgA260/A280 1.80–1.95Endotoxin level Preparation time 100 min/2 prepsBinding capacity 500 µgNucleoBond® AX 500 Columns, buffers EF, RNase A, NucleoBond® Folded Filters XL 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond AX 500 Columns, NucleoBond Folded Filters, buffers, RNase A

经典离子交换柱，用于纯化无内毒素级别纯度的质粒DNA。可纯化高分子量质粒。载量2000 µg• Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells• Patented endotoxin removal by additional washing step – no extra ice incubation necessary• Fast and easy clarification of lysates by NucleoBond® Folded Filters, no centrifugation required, no shearing forces• NucleoBond® Finalizers available as separate tool to avoid time consuming centrifugation for plasmid precipitation• Transfection-grade plasmid DNA due to patented anion-exchange technology• Separate binding columns of NucleoBond® PC kits available as NucleoBond® AX columnsTechnology Anion-exchange chromatography Gravity-flow columnsFormat MegaLysate clarification: Bottle top filtersSample material 150–500 mLVector size Typical yield 1700–2000 µgA260/A280 1.80–1.95Endotoxin level Preparation time 150 min/2 prepsBinding capacity 2000 µgNucleoBond® AX 2000 Columns, buffers EF, RNase A, NucleoBond® Bottle Top Filters 5 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond AX 2000 Columns, NucleoBond Bottle Top Filters Type 1, buffers, RNase A

新一代离子交换柱，超快获得高产量无内毒素级别超纯质粒。可纯化高分子量质粒。Column filter同步完成上清过滤和质粒吸附。只需105 min，产量翻倍,1000 ug以上。• Plasmid DNA with less than 0.05 EU/μg for transfection of highly sensitive cells• Patented endotoxin removal by additional washing step – no extra ice incubation necessary• Optimized column design: Midi prep in approximately 45 min, Maxi prep in 50 min• Column filter included: high filter flow-rates, parallel lysate clearing and loading onto the column• Improved silica material: typical yields of 250 μg (Midi) and 1000 μg (Maxi)Technology Anion-exchange chromatography Format Midi gravity flow columnsLysate clarification Column filtersSample material Vector size Typical yield > 250 µgA260/A280 1.8–1.95Endotoxin level Isopropanol precipitation：CentrifugationPreparation time 105 min/4 prepsNucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A

新一代离子交换柱，超快获得高产量无内毒素级别超纯质粒。Column filter同步完成上清过滤和质粒吸附。NucleoBond® Finalizers滤器快速浓缩沉淀质粒，无需离心。只需50 min，产量翻倍,1000 ug以上。• Plasmid DNA with less than 0.05 EU/μg for transfection of highly sensitive cells• Patented endotoxin removal by additional washing step – no extra ice incubation necessary• Optimized column design: Midi prep in approximately 45 min, Maxi prep in 50 min• Column filter included: high filter flow-rates, parallel lysate clearing and loading onto the column• Improved silica material: typical yields of 250 μg (Midi) and 1000 μg (Maxi)• NucleoBond® Xtra Plus EF kits contain NucleoBond® Finalizers to speed up DNA precipitationTechnology Anion-exchange chromatography Format Midi gravity flow columnsLysate clarification Column filtersSample material Typical yield > 1000 µgA260/A280 1.8–1.95Endotoxin level Isopropanol precipitation：NucleoBond® FinalizersPreparation time： 50 min/4 prepsNucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizer Large, buffers, RNase A 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, NucleoBond Finalizers Large, syringes, buffers, RNase A

经典离子交换柱，用于纯化无内毒素级别纯度的质粒DNA。可纯化高分子量质粒。载量10000 µg• Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells• Patented endotoxin removal by additional washing step – no extra ice incubation necessary• Fast and easy clarification of lysates by NucleoBond® Folded Filters, no centrifugation required, no shearing forces• NucleoBond® Finalizers available as separate tool to avoid time consuming centrifugation for plasmid precipitation• Transfection-grade plasmid DNA due to patented anion-exchange technology• Separate binding columns of NucleoBond® PC kits available as NucleoBond® AX columnsTechnology Anion-exchange chromatography Gravity-flow columnsFormat Giga，Lysate clarification: Bottle top filtersSample material 500–2000 mLVector size Typical yield 5000–9000 µgA260/A280 1.80–1.95Endotoxin level Preparation time 180 min/2 prepsBinding capacity 10000 µgNucleoBond® AX 10000 Columns, buffers EF, RNase A, NucleoBond® Bottle Top Filters 5 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond AX 10000 Columns, NucleoBond Bottle Top Filters Type 2, buffers, RNase A

96孔板高通量纯化无内毒素的转染级别纯度质粒。5ml培养物可获得高达50ug产物。 High-throughput isolation of transfection-grade,endotoxin-free plasmid DNA in 96-well format • Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells• Patented endotoxin removal by additional washing step • Processing of up to 5 mL bacterial culture• NucleoBond® Filter Plate for convenient filtration of bacterial lysates• Processing under vacuum or by centrifugation• Innovative MN Wash Plate minimizes risk of cross-contamination• NucleoBond® Finalizer Plate to speed up DNA precipitationTechnology Anion-exchange chromatographyFormat 96-well platesProcessing Manual Lysate clarification 96-well filter platesSample material 1–5 mL E. coli cultureVector size Typical yield 2–4 μg (1.5 mL LB/TB in 96-well culture plates) 10–50 µg (5 mL LB/TB in glass tubes)A260/A280 1.80–1.95Elution volume 100–200 μLPreparation time 120 min/plateBinding capacity 50 µg NucleoBond® Xtra EF Plate, NucleoBond® Finalizer Plate, NucleoBond® Filter Plate, Culture Plate, MN Wash Plate, Elution Plate U-bottom, Gas-permeable Foil, Self-adgering Foil, buffers, RNase A1 x 96 preps for the isolation of endotoxin free plasmid DNA - NucleoBond Filter Plate, NucleoBond Xtra EF Plate, NucleoBond Finalizer Plate, MN Wash Plate, Square-well Block, Culture Plate, Gas-permeable Foil, Elution Plate u-bottom, Self-adhering Foil, buffers, RNase A

新一代离子交换柱，超快获得高产量无内毒素级别超纯质粒。可纯化高分子量质粒。Column filter同步完成上清过滤和质粒吸附。只需100 min，产量翻倍,250ug以上。• Plasmid DNA with less than 0.05 EU/μg for transfection of highly sensitive cells• Patented endotoxin removal by additional washing step – no extra ice incubation necessary• Optimized column design: Midi prep in approximately 45 min, Maxi prep in 50 min• Column filter included: high filter flow-rates, parallel lysate clearing and loading onto the column• Improved silica material: typical yields of 250 μg (Midi) and 1000 μg (Maxi)Technology Anion-exchange chromatography Format Midi gravity flow columnsLysate clarification Column filtersSample material Vector size Typical yield > 250 µgA260/A280 1.8–1.95Endotoxin level Isopropanol precipitation：CentrifugationPreparation time 100 min/4 prepsNucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A

新一代离子交换柱，超快获得高产量无内毒素级别超纯质粒。Column filter同步完成上清过滤和质粒吸附。NucleoBond® Finalizers滤器快速浓缩沉淀质粒，无需离心。只需45 min，产量翻倍,250ug以上。• Plasmid DNA with less than 0.05 EU/μg for transfection of highly sensitive cells• Patented endotoxin removal by additional washing step – no extra ice incubation necessary• Optimized column design: Midi prep in approximately 45 min, Maxi prep in 50 min• Column filter included: high filter flow-rates, parallel lysate clearing and loading onto the column• Improved silica material: typical yields of 250 μg (Midi) and 1000 μg (Maxi)• NucleoBond® Xtra Plus EF kits contain NucleoBond® Finalizers to speed up DNA precipitationTechnology Anion-exchange chromatography Format Midi gravity flow columnsLysate clarification Column filtersSample material Typical yield > 250 µgA260/A280 1.8–1.95Endotoxin level Isopropanol precipitation：NucleoBond® FinalizersPreparation time： 45 min/4 prepsNucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizer, buffers, RNase A 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, NucleoBond Finalizers, syringes, buffers, RNase A

经典离子交换柱，用于纯化无内毒素级别纯度的质粒DNA。可纯化高分子量质粒。生产级，载量100mg• Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells• Patented endotoxin removal by additional washing step – no extra ice incubation necessary• Fast and easy clarification of lysates by NucleoBond® Folded Filters, no centrifugation required, no shearing forces• NucleoBond® Finalizers available as separate tool to avoid time consuming centrifugation for plasmid precipitation• Transfection-grade plasmid DNA due to patented anion-exchange technology• Separate binding columns of NucleoBond® PC kits available as NucleoBond® AX columnsTechnology Anion-exchange chromatography Gravity-flow columnsFormat Preparative Scale(Compatible with e.g., HPLC, FPLC)Lysate clarification: Folded filters, sieving fabricSample material 5–20 LVector size Typical yield 80–100 mg A260/A280 1.80–1.95Endotoxin level Preparation time 20 h/prepBinding capacity 100 mg1 NucleoBond® AX Prep 100 Column,3 NucleoBond® AX 100 Columns, buffers EF, RNase A, Sieving Fabric, NucleoBond® Folded Filters 1 prep for large-scale isolation of endotoxin-free plasmid DNA - NucleoBond AX Prep 100 Column, NucleoBond AX 100 Columns, NucleoBond Folded Filters, buffers, RNase A

新一代离子交换柱，超快获得高产量无内毒素级别超纯质粒。可纯化高分子量质粒。Column filter同步完成上清过滤和质粒吸附。只需105 min，产量翻倍,1000 ug以上。• Plasmid DNA with less than 0.05 EU/μg for transfection of highly sensitive cells• Patented endotoxin removal by additional washing step – no extra ice incubation necessary• Optimized column design: Midi prep in approximately 45 min, Maxi prep in 50 min• Column filter included: high filter flow-rates, parallel lysate clearing and loading onto the column• Improved silica material: typical yields of 250 μg (Midi) and 1000 μg (Maxi)Technology Anion-exchange chromatography Format Midi gravity flow columnsLysate clarification Column filtersSample material Vector size Typical yield > 250 µgA260/A280 1.8–1.95Endotoxin level Isopropanol precipitation：CentrifugationPreparation time 105 min/4 prepsNucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A

96孔板高通量纯化无内毒素的转染级别纯度质粒。5ml培养物可获得高达50ug产物。 High-throughput isolation of transfection-grade,endotoxin-free plasmid DNA in 96-well format • Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells• Patented endotoxin removal by additional washing step • Processing of up to 5 mL bacterial culture• NucleoBond® Filter Plate for convenient filtration of bacterial lysates• Processing under vacuum or by centrifugation• Innovative MN Wash Plate minimizes risk of cross-contamination• NucleoBond® Finalizer Plate to speed up DNA precipitationTechnology Anion-exchange chromatographyFormat 96-well platesProcessing Manual Lysate clarification 96-well filter platesSample material 1–5 mL E. coli cultureVector size Typical yield 2–4 μg (1.5 mL LB/TB in 96-well culture plates) 10–50 µg (5 mL LB/TB in glass tubes)A260/A280 1.80–1.95Elution volume 100–200 μLPreparation time 120 min/plateBinding capacity 50 µg NucleoBond® Xtra EF Plate, NucleoBond® Finalizer Plate, NucleoBond® Filter Plate, Culture Plate, MN Wash Plate, Elution Plate U-bottom, Gas-permeable Foil, Self-adgering Foil, buffers, RNase A4 x 96 preps for the isolation of endotoxin free plasmid DNA - NucleoBond Filter Plate, NucleoBond Xtra EF Plate, NucleoBond Finalizer Plate, MN Wash Plate, Square-well Block, Culture Plate, Gas-permeable Foil, Elution Plate u-bottom, Self-adhering Foil, buffers, RNase A

新一代离子交换柱，超快获得高产量无内毒素级别超纯质粒。Column filter同步完成上清过滤和质粒吸附。NucleoBond® Finalizers滤器快速浓缩沉淀质粒，无需离心。只需50 min，产量翻倍,1000 ug以上。• Plasmid DNA with less than 0.05 EU/μg for transfection of highly sensitive cells• Patented endotoxin removal by additional washing step – no extra ice incubation necessary• Optimized column design: Midi prep in approximately 45 min, Maxi prep in 50 min• Column filter included: high filter flow-rates, parallel lysate clearing and loading onto the column• Improved silica material: typical yields of 250 μg (Midi) and 1000 μg (Maxi)• NucleoBond® Xtra Plus EF kits contain NucleoBond® Finalizers to speed up DNA precipitationTechnology Anion-exchange chromatography Format Midi gravity flow columnsLysate clarification Column filtersSample material Typical yield > 1000 µgA260/A280 1.8–1.95Endotoxin level Isopropanol precipitation：NucleoBond® FinalizersPreparation time： 50 min/4 prepsNucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizer Large, buffers, RNase A 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, NucleoBond Finalizers Large, syringes, buffers, RNase A