新一代离子交换柱，超快获得高产量无内毒素级别超纯质粒。Column filter同步完成上清过滤和质粒吸附。NucleoBond® Finalizers滤器快速浓缩沉淀质粒，无需离心。只需45 min，产量翻倍,250ug以上。 • Plasmid DNA with less than 0.05 EU/μg for transfection of highly sensitive cells • Patented endotoxin removal by additional washing step – no extra ice incubation necessary • Optimized column design: Midi prep in approximately 45 min, Maxi prep in 50 min • Column filter included: high filter flow-rates, parallel lysate clearing and loading onto the column • Improved silica material: typical yields of 250 μg (Midi) and 1000 μg (Maxi) • NucleoBond® Xtra Plus EF kits contain NucleoBond® Finalizers to speed up DNA precipitation Technology Anion-exchange chromatography Format Midi gravity flow columns Lysate clarification Column filters Sample material < 200 mL (high copy)；< 400 mL (low copy) Typical yield > 250 µg A260/A280 1.8–1.95 Endotoxin level < 0.05 EU/µg DNA Isopropanol precipitation：NucleoBond® Finalizers Preparation time： 45 min/4 preps NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizer, buffers, RNase A 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, NucleoBond Finalizers, syringes, buffers, RNase A
经典离子交换柱，用于纯化无内毒素级别纯度的质粒DNA。可纯化高分子量质粒。载量500µg • Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells • Patented endotoxin removal by additional washing step – no extra ice incubation necessary • Fast and easy clarification of lysates by NucleoBond® Folded Filters, no centrifugation required, no shearing forces • NucleoBond® Finalizers available as separate tool to avoid time consuming centrifugation for plasmid precipitation • Transfection-grade plasmid DNA due to patented anion-exchange technology • Separate binding columns of NucleoBond® PC kits available as NucleoBond® AX columns Technology Anion-exchange chromatography Gravity-flow columns Format Maxi Lysate clarification: Folded filters Sample material 30–150 mL Vector size < 300 kbp Typical yield 450–520 µg A260/A280 1.80–1.95 Endotoxin level < 0.1 EU/µg Preparation time 100 min/2 preps Binding capacity 500 µg NucleoBond® AX 500 Columns, buffers EF, RNase A, NucleoBond® Folded Filters XL 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond AX 500 Columns, NucleoBond Folded Filters, buffers, RNase A
经典离子交换柱，用于纯化无内毒素级别纯度的质粒DNA。可纯化高分子量质粒。载量2000 µg • Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells • Patented endotoxin removal by additional washing step – no extra ice incubation necessary • Fast and easy clarification of lysates by NucleoBond® Folded Filters, no centrifugation required, no shearing forces • NucleoBond® Finalizers available as separate tool to avoid time consuming centrifugation for plasmid precipitation • Transfection-grade plasmid DNA due to patented anion-exchange technology • Separate binding columns of NucleoBond® PC kits available as NucleoBond® AX columns Technology Anion-exchange chromatography Gravity-flow columns Format Mega Lysate clarification: Bottle top filters Sample material 150–500 mL Vector size < 300 kbp Typical yield 1700–2000 µg A260/A280 1.80–1.95 Endotoxin level < 0.1 EU/µg Preparation time 150 min/2 preps Binding capacity 2000 µg NucleoBond® AX 2000 Columns, buffers EF, RNase A, NucleoBond® Bottle Top Filters 5 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond AX 2000 Columns, NucleoBond Bottle Top Filters Type 1, buffers, RNase A
经典离子交换柱，用于纯化无内毒素级别纯度的质粒DNA。可纯化高分子量质粒。载量10000 µg • Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells • Patented endotoxin removal by additional washing step – no extra ice incubation necessary • Fast and easy clarification of lysates by NucleoBond® Folded Filters, no centrifugation required, no shearing forces • NucleoBond® Finalizers available as separate tool to avoid time consuming centrifugation for plasmid precipitation • Transfection-grade plasmid DNA due to patented anion-exchange technology • Separate binding columns of NucleoBond® PC kits available as NucleoBond® AX columns Technology Anion-exchange chromatography Gravity-flow columns Format Giga， Lysate clarification: Bottle top filters Sample material 500–2000 mL Vector size < 300 kbp Typical yield 5000–9000 µg A260/A280 1.80–1.95 Endotoxin level < 0.1 EU/µg Preparation time 180 min/2 preps Binding capacity 10000 µg NucleoBond® AX 10000 Columns, buffers EF, RNase A, NucleoBond® Bottle Top Filters 5 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond AX 10000 Columns, NucleoBond Bottle Top Filters Type 2, buffers, RNase A
96孔板高通量纯化无内毒素的转染级别纯度质粒。5ml培养物可获得高达50ug产物。 High-throughput isolation of transfection-grade,endotoxin-free plasmid DNA in 96-well format • Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells • Patented endotoxin removal by additional washing step • Processing of up to 5 mL bacterial culture • NucleoBond® Filter Plate for convenient filtration of bacterial lysates • Processing under vacuum or by centrifugation • Innovative MN Wash Plate minimizes risk of cross-contamination • NucleoBond® Finalizer Plate to speed up DNA precipitation Technology Anion-exchange chromatography Format 96-well plates Processing Manual Lysate clarification 96-well filter plates Sample material 1–5 mL E. coli culture Vector size < 15 kbp，< 300 kbp (without NucleoBond® Finalizer Plate) Typical yield 2–4 μg (1.5 mL LB/TB in 96-well culture plates) 10–50 µg (5 mL LB/TB in glass tubes) A260/A280 1.80–1.95 Elution volume 100–200 μL Preparation time 120 min/plate Binding capacity 50 µg NucleoBond® Xtra EF Plate, NucleoBond® Finalizer Plate, NucleoBond® Filter Plate, Culture Plate, MN Wash Plate, Elution Plate U-bottom, Gas-permeable Foil, Self-adgering Foil, buffers, RNase A 1 x 96 preps for the isolation of endotoxin free plasmid DNA - NucleoBond Filter Plate, NucleoBond Xtra EF Plate, NucleoBond Finalizer Plate, MN Wash Plate, Square-well Block, Culture Plate, Gas-permeable Foil, Elution Plate u-bottom, Self-adhering Foil, buffers, RNase A
新一代离子交换柱，超快获得高产量无内毒素级别超纯质粒。Column filter同步完成上清过滤和质粒吸附。NucleoBond® Finalizers滤器快速浓缩沉淀质粒，无需离心。只需45 min，产量翻倍,250ug以上。 • Plasmid DNA with less than 0.05 EU/μg for transfection of highly sensitive cells • Patented endotoxin removal by additional washing step – no extra ice incubation necessary • Optimized column design: Midi prep in approximately 45 min, Maxi prep in 50 min • Column filter included: high filter flow-rates, parallel lysate clearing and loading onto the column • Improved silica material: typical yields of 250 μg (Midi) and 1000 μg (Maxi) • NucleoBond® Xtra Plus EF kits contain NucleoBond® Finalizers to speed up DNA precipitation Technology Anion-exchange chromatography Format Midi gravity flow columns Lysate clarification Column filters Sample material < 200 mL (high copy)；< 400 mL (low copy) Typical yield > 250 µg A260/A280 1.8–1.95 Endotoxin level < 0.05 EU/µg DNA Isopropanol precipitation：NucleoBond® Finalizers Preparation time： 45 min/4 preps NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizer, buffers, RNase A 50 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, NucleoBond Finalizers, syringes, buffers, RNase A
经典离子交换柱，用于纯化无内毒素级别纯度的质粒DNA。可纯化高分子量质粒。生产级，载量100mg • Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells • Patented endotoxin removal by additional washing step – no extra ice incubation necessary • Fast and easy clarification of lysates by NucleoBond® Folded Filters, no centrifugation required, no shearing forces • NucleoBond® Finalizers available as separate tool to avoid time consuming centrifugation for plasmid precipitation • Transfection-grade plasmid DNA due to patented anion-exchange technology • Separate binding columns of NucleoBond® PC kits available as NucleoBond® AX columns Technology Anion-exchange chromatography Gravity-flow columns Format Preparative Scale(Compatible with e.g., HPLC, FPLC) Lysate clarification: Folded filters, sieving fabric Sample material 5–20 L Vector size < 300 kbp Typical yield 80–100 mg A260/A280 1.80–1.95 Endotoxin level < 0.1 EU/µg Preparation time 20 h/prep Binding capacity 100 mg 1 NucleoBond® AX Prep 100 Column,3 NucleoBond® AX 100 Columns, buffers EF, RNase A, Sieving Fabric, NucleoBond® Folded Filters 1 prep for large-scale isolation of endotoxin-free plasmid DNA - NucleoBond AX Prep 100 Column, NucleoBond AX 100 Columns, NucleoBond Folded Filters, buffers, RNase A
96孔板高通量纯化无内毒素的转染级别纯度质粒。5ml培养物可获得高达50ug产物。 High-throughput isolation of transfection-grade,endotoxin-free plasmid DNA in 96-well format • Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells • Patented endotoxin removal by additional washing step • Processing of up to 5 mL bacterial culture • NucleoBond® Filter Plate for convenient filtration of bacterial lysates • Processing under vacuum or by centrifugation • Innovative MN Wash Plate minimizes risk of cross-contamination • NucleoBond® Finalizer Plate to speed up DNA precipitation Technology Anion-exchange chromatography Format 96-well plates Processing Manual Lysate clarification 96-well filter plates Sample material 1–5 mL E. coli culture Vector size < 15 kbp，< 300 kbp (without NucleoBond® Finalizer Plate) Typical yield 2–4 μg (1.5 mL LB/TB in 96-well culture plates) 10–50 µg (5 mL LB/TB in glass tubes) A260/A280 1.80–1.95 Elution volume 100–200 μL Preparation time 120 min/plate Binding capacity 50 µg NucleoBond® Xtra EF Plate, NucleoBond® Finalizer Plate, NucleoBond® Filter Plate, Culture Plate, MN Wash Plate, Elution Plate U-bottom, Gas-permeable Foil, Self-adgering Foil, buffers, RNase A 4 x 96 preps for the isolation of endotoxin free plasmid DNA - NucleoBond Filter Plate, NucleoBond Xtra EF Plate, NucleoBond Finalizer Plate, MN Wash Plate, Square-well Block, Culture Plate, Gas-permeable Foil, Elution Plate u-bottom, Self-adhering Foil, buffers, RNase A
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