The silica-matrix based kit for gel extraction without columns • Batch binding procedure • High binding capacity even for very small fragments ≥ 20 bp • Large DNA fragments are neither sheared nor degraded
Technology Silica-matrix technology Format Silica bead suspension Sample material < 200 mg agarose gel Fragment size 20 bp–approx. 50 kbp Typical recovery 50–90% A260/A280 1.7–1.9 Elution volume 20–50 µL Preparation time 60 min/6 preps Binding capacity 6 µg/10 µL suspension
Applications • Extraction of DNA from agarose gels • Support protocol for concentration and desalination of reaction mixtures • Typical downstream applications: cloning, sequencing, PCR, restriction analysis NucleoTrap® Suspension, buffers 10 preps for gel extraction - NucleoTrap suspension, buffers pack of 10
高分子量DNA的后继纯化浓缩。快速高效回收100 bp–50 kb DNA.不含裂解液。 Post clean-up and concentration of large sized DNA • Effective post clean-up and concentration of large-sized DNA for successful downstream applications • High recovery rates up to 90 % for DNA fragments 100 bp–50 kbp • Concentrate your DNA sample easily and faster compared to microdialysis filtrations units • 15 min/10 preps – time-saving procedure
Technology: Silica-membrane technology Format: Mini spin columns Sample material: < 150 µL aqueous DNA solution (< 25 µg DNA) Fragment size: 100 bp–approx. 50 kbp Typical recovery: 80–90 % A260/A280: 1.8–1.9 Elution volume: 50–100 µL Preparation time: 15 min/10 preps Binding capacity: 50 µg Applications • Clean-up and concentration of pre-purified DNA (organic extractions) • Clean-up and concentration of DNA from enzymatic reactions • Not recommended for gel or PCR clean-up • Typical downstream applications: qPCR, enzymatic reactions, Southern blotting, STR amplification NucleoSpin® gDNA Clean-up Columns,Collection Tubes (2 mL), buffers 10 preps for clean-up of genomic DNA in aqueous solutions - NucleoSpin gDNA Clean-up Columns, Collection Tubes, buffers
微量的DNA大片段快速回收浓缩，洗脱体积仅为6–10 µL Post clean-up and concentration of small amounts of large DNA fragments • Efficient post clean-up of large DNA fragments for successful downstream applications • Recommended for small DNA quantities extracted from, e.g., forensic samples • Unique XS spin column for low elution volume (> 6 µL) – highly concentrated DNA • Easier and faster than microdialysis filtration units • 20 min/6 preps – time-saving procedure
高分子量DNA的后继纯化浓缩。快速高效回收100 bp–50 kb DNA.不含裂解液。 Post clean-up and concentration of large sized DNA • Effective post clean-up and concentration of large-sized DNA for successful downstream applications • High recovery rates up to 90 % for DNA fragments 100 bp–50 kbp • Concentrate your DNA sample easily and faster compared to microdialysis filtrations units • 15 min/10 preps – time-saving procedure
Technology: Silica-membrane technology Format: Mini spin columns Sample material: < 150 µL aqueous DNA solution (< 25 µg DNA) Fragment size: 100 bp–approx. 50 kbp Typical recovery: 80–90 % A260/A280: 1.8–1.9 Elution volume: 50–100 µL Preparation time: 15 min/10 preps Binding capacity: 50 µg Applications • Clean-up and concentration of pre-purified DNA (organic extractions) • Clean-up and concentration of DNA from enzymatic reactions • Not recommended for gel or PCR clean-up • Typical downstream applications: qPCR, enzymatic reactions, Southern blotting, STR amplification NucleoSpin® gDNA Clean-up Columns,Collection Tubes (2 mL), buffers 50 preps for clean-up of genomic DNA in aqueous solutions - NucleoSpin gDNA Clean-up Columns, Collection Tubes, buffers
微量的DNA大片段快速回收浓缩，洗脱体积仅为6–10 µL Post clean-up and concentration of small amounts of large DNA fragments • Efficient post clean-up of large DNA fragments for successful downstream applications • Recommended for small DNA quantities extracted from, e.g., forensic samples • Unique XS spin column for low elution volume (> 6 µL) – highly concentrated DNA • Easier and faster than microdialysis filtration units • 20 min/6 preps – time-saving procedure
The silica-matrix based kit for gel extraction without columns • Batch binding procedure • High binding capacity even for very small fragments ≥ 20 bp • Large DNA fragments are neither sheared nor degraded
Technology Silica-matrix technology Format Silica bead suspension Sample material < 200 mg agarose gel Fragment size 20 bp–approx. 50 kbp Typical recovery 50–90% A260/A280 1.7–1.9 Elution volume 20–50 µL Preparation time 60 min/6 preps Binding capacity 6 µg/10 µL suspension
Applications • Extraction of DNA from agarose gels • Support protocol for concentration and desalination of reaction mixtures • Typical downstream applications: cloning, sequencing, PCR, restriction analysis
NucleoTrap Suspension for 100 preps for gel extraction pack of 1 mL
The silica-matrix based kit for gel extraction without columns • Batch binding procedure • High binding capacity even for very small fragments ≥ 20 bp • Large DNA fragments are neither sheared nor degraded
Technology Silica-matrix technology Format Silica bead suspension Sample material < 200 mg agarose gel Fragment size 20 bp–approx. 50 kbp Typical recovery 50–90% A260/A280 1.7–1.9 Elution volume 20–50 µL Preparation time 60 min/6 preps Binding capacity 6 µg/10 µL suspension
Applications • Extraction of DNA from agarose gels • Support protocol for concentration and desalination of reaction mixtures • Typical downstream applications: cloning, sequencing, PCR, restriction analysis NucleoTrap® Suspension, buffers 100 preps for gel extraction - NucleoTrap Suspension, buffers pack of 100
高分子量DNA的后继纯化浓缩。快速高效回收100 bp–50 kb DNA.不含裂解液。 Post clean-up and concentration of large sized DNA • Effective post clean-up and concentration of large-sized DNA for successful downstream applications • High recovery rates up to 90 % for DNA fragments 100 bp–50 kbp • Concentrate your DNA sample easily and faster compared to microdialysis filtrations units • 15 min/10 preps – time-saving procedure
Technology: Silica-membrane technology Format: Mini spin columns Sample material: < 150 µL aqueous DNA solution (< 25 µg DNA) Fragment size: 100 bp–approx. 50 kbp Typical recovery: 80–90 % A260/A280: 1.8–1.9 Elution volume: 50–100 µL Preparation time: 15 min/10 preps Binding capacity: 50 µg Applications • Clean-up and concentration of pre-purified DNA (organic extractions) • Clean-up and concentration of DNA from enzymatic reactions • Not recommended for gel or PCR clean-up • Typical downstream applications: qPCR, enzymatic reactions, Southern blotting, STR amplification NucleoSpin® gDNA Clean-up Columns,Collection Tubes (2 mL), buffers 250 preps for clean-up of genomic DNA in aqueous solutions - NucleoSpin gDNA Clean-up Columns, Collection Tubes, buffers
微量的DNA大片段快速回收浓缩，洗脱体积仅为6–10 µL Post clean-up and concentration of small amounts of large DNA fragments • Efficient post clean-up of large DNA fragments for successful downstream applications • Recommended for small DNA quantities extracted from, e.g., forensic samples • Unique XS spin column for low elution volume (> 6 µL) – highly concentrated DNA • Easier and faster than microdialysis filtration units • 20 min/6 preps – time-saving procedure
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