20 mlreduced-serum medium suitable for addition to siRNA suspension and siRNA transfection reagent, immediately prior to cell transfectionModification of Eagle's Minimal Essential Medium, buffered with HEPES and sodium bicarbonate, and supplemented with hypoxanthine, thymidine, sodium pyruvate, L-glutamine, trace elements and growth factors; protein level is minimal with insulin and transferrin being the only protein supplements; phenol red included at a reduced concentration as a pH indicatorstore at 4° C; light sensitive
20 mlReduced-serum medium suitable for transfections with DNA PlasmidsRecommended for use with Plasmid Transfection Reagent (sc-108061) and suspension of DNA for transfection including: shRNA Plasmids, CRISPR/Cas9 KO Plasmids, HDR Plasmids or Cre VectorModification of Eagle's Minimal Essential Medium, buffered with HEPES and sodium bicarbonate, and supplemented with hypoxanthine, thymidine, sodium pyruvate, L-glutamine, trace elements and growth factors; protein level is minimal with insulin and transferrin being the only protein supplements; phenol red included at a reduced concentration as a pH indicatorStore at 4° C; light sensitive
1 ml; 10mg/ml in sterile waterhighly efficient infection reagent used to introduce retroviral vectors into mammalian cellsalso known as hexamethrine bromide, 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromidePolybrene® is a registered trademark of Abbott Laboratories Corp.Store at -20º C
0.2 ml; Sufficient for 50-100 transfections (per well in a 6-well plate)Enables highly efficient DNA transfection in a variety of cell lines including CHO-K1, COS, LNCaP, NIH/3T3, 293, T24, C2C12, SF-9, primary human keratinocytes, primary aortic smooth muscle, primary rabbit myoblasts and human bone marrow endothelial cells (HBMEC)Recommended for use with shRNA PlasmidsEffective in both serum-containing medium and serum-free medium, offering high efficiencies with minimal cytotoxicitystore at 4° C; DO NOT FREEZE; protect from light
0.3 ml; sufficient for 50-100 transfections (using 12-well plates)enables highly efficient siRNA transfection in a variety of cell lines including HeLa, A549, Jurkat and NIH/3T3Effective in both serum-containing medium and serum-free medium, offering high efficiencies with minimal cytotoxicitystore at 4° C; do not freeze; protect from light
20 µg 纯化的即用型的质粒DNA,更多可供20次转染Enables highly efficient DNA transfection in a variety of cell lines including CHO-K1, COS, LNCaP, NIH/3T3, 293, T24, C2C12, SF-9, primary human keratinocytes, primary aortic smooth muscle, primary rabbit myoblasts and human bone marrow endothelial cells (HBMEC)Recommended for use with CRISPR/Cas9 KO Plasmids, HDR Plasmids and Cre Vectorstore at 4° C; DO NOT FREEZE; protect from light