NEBNext® dsDNA Fragmentase® Reaction Buffer v2, 6.0 ml NEBNext® dsDNA片段化酶反应缓冲液，10XBuffer，1X反应体系即可确保酶的更适活性。

NEBNext超保真 2X PCR预混液专门针对二代测序文库制备中的PCR扩增反应进行了优化，不论GC含量多少，该产品都能够进行稳定、高保真的扩增。特殊的预混液中包含了错配率极低的 Q5 超保真 DNA 聚合酶、优化的缓冲液和反应增强剂，适合大多数模板的扩增，包括高 GC 含量的模板。该预混液的缓冲液组份针对高 GC 含量的模板甚至是二代测序文库都可提供超强的扩增。

为新一代测序文库扩增优化的高性能超保真扩增酶预混液，超过常规Taq酶100倍的超高保真度，超强扩增能力，可用于高GC含量模板，是NGS文库构建的理想之选。Specifically optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries, regardless of GC content. The polymerase component of the master mix
The NEBNext Q5 Hot Start HiFi PCR Master Mix is specifically optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries, regardless of GC content. The polymerase component of the master mix, Q5 High-Fidelity DNA Polymerase, is a novel thermostable DNA polymerase that possesses 3´→5´ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5 also has an ultra-low error rate (> 100-fold lower than that of Taq DNA Polymerase and ~12-fold lower than that of Pyrococcus furiosus (Pfu) DNA Polymerase). The buffer component of the master mix has been optimized for robust amplification, even with GC-rich amplicons and offers enhanced compatibility with a variety of beads used in typical NGS workflows. These features make the NEBNext Q5 Hot Start HiFi PCR Master Mix ideal for NGS library construction. This convenient 2X master mix contains dNTPs, Mg++ and a proprietary buffer, and requires only the addition of primers and DNA template for robust amplification. The inclusion of the hot start aptamer allows convenient room temperature reaction set up. Applications Next generation sequencing library construction High-fidelity PCR Difficult amplification High-throughput PCR

二代测序文库制备,应用：制备DNA文库,Illumina平台文库制备；NEBNext Ultra II Q5? Master Mix是Q5DNA聚合酶的新配方,大大提高了文库扩增后的一致性,包括GC富集区的优化。 Master Mix的主要成分，Q5?高保真DNA聚合酶,是一种新型耐热性的DNA聚合酶,具有3?→5?核酸外切酶活性。NEBNext Ultra II Q5 Master Mix, 50 rxns

NEBNext 超快速末端修复/加 dA 尾模块   

DNA模块产品,二代测序文库制备；适用于:制备DNA文库,Illumina®平台文库制备，用于新一代测序的NEBNext®试剂

为新一代测序文库扩增优化的高性能超保真扩增酶预混液，超过常规Taq酶100倍的超高保真度，超强扩增能力，可用于高GC含量模板，是NGS文库构建的理想之选。Specifically optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries, regardless of GC content. The polymerase component of the master mix
The NEBNext Q5 Hot Start HiFi PCR Master Mix is specifically optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries, regardless of GC content. The polymerase component of the master mix, Q5 High-Fidelity DNA Polymerase, is a novel thermostable DNA polymerase that possesses 3´→5´ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5 also has an ultra-low error rate (> 100-fold lower than that of Taq DNA Polymerase and ~12-fold lower than that of Pyrococcus furiosus (Pfu) DNA Polymerase). The buffer component of the master mix has been optimized for robust amplification, even with GC-rich amplicons and offers enhanced compatibility with a variety of beads used in typical NGS workflows. These features make the NEBNext Q5 Hot Start HiFi PCR Master Mix ideal for NGS library construction. This convenient 2X master mix contains dNTPs, Mg++ and a proprietary buffer, and requires only the addition of primers and DNA template for robust amplification. The inclusion of the hot start aptamer allows convenient room temperature reaction set up. Applications Next generation sequencing library construction High-fidelity PCR Difficult amplification High-throughput PCR

NEBNext超保真 2X PCR预混液专门针对二代测序文库制备中的PCR扩增反应进行了优化，不论GC含量多少，该产品都能够进行稳定、高保真的扩增。特殊的预混液中包含了错配率极低的 Q5 超保真 DNA 聚合酶、优化的缓冲液和反应增强剂，适合大多数模板的扩增，包括高 GC 含量的模板。该预混液的缓冲液组份针对高 GC 含量的模板甚至是二代测序文库都可提供超强的扩增。

二代测序文库制备,应用：制备DNA文库,Illumina平台文库制备；NEBNext Ultra II Q5? Master Mix是Q5DNA聚合酶的新配方,大大提高了文库扩增后的一致性,包括GC富集区的优化。 Master Mix的主要成分，Q5?高保真DNA聚合酶,是一种新型耐热性的DNA聚合酶,具有3?→5?核酸外切酶活性。NEBNext Ultra II Q5 Master Mix, 250 rxns

NEBNext 超快速连接模块  

DNA模块,新一代测序文库制备产品；适用于:制备DNA库,Illumina®平台文库制备,新一代测序样品制备的NEBNext®试剂

NEB二代测序文库制备产品；适用于:制备DNA库,Illumina®平台文库制备,二代测序样品制备的NEBNext®试剂；新一代的NEBNext试剂使用快速、流线型、可自动化的工作流程,减少PCR循环次数,同提高GC含量。同时也兼容PCR-free；NEBNext Ultra II DNA Library Prep Kit for Illumina, 24 rxns

NEBNext 微生物 DNA 富集试剂盒；该试剂盒适用于从甲基化宿主 DNA 的样品中（包括人源样品）富集微生物 DNA，主要是通过选择性结合并移除带有 CpG 甲基化的宿主 DNA 来达到富集微生物 DNA 的目的 The NEBNext Microbiome DNA Enrichment Kit facilitates enrichment of microbial DNA from samples containing methylated host DNA (including human), by selective binding and removal of the CpG-methylated host DNA. Importantly, microbial diversity remains intact after enrichment.

NEBNext 超快速末端修复/加 dA 尾模块   

DNA模块产品,二代测序文库制备；适用于:制备DNA文库,Illumina®平台文库制备，用于新一代测序的NEBNext®试剂

NEBNext DNA快速文库制备试剂盒-Ion Torrent，产品可用于快速操作和低样本起始量，简单方便的剂型，更高性价比  

NEBNext DNA快速片段化&文库制备试剂盒-Ion Torrent，产品可用于快速操作和低样本起始量，简单方便的剂型，更高性价比  

靶向富集，配合 NGS 技术，使对基因组中目标区域高通量、高深度的测序成为可能。 NEBNext Direct 是一种全新的，基于杂交的捕获方法，与传统的液相杂交及多重 PCR 方法相比有明显的优势。 在 NEBNext Direct 靶向富集方法中（图1），片段化的 DNA 迅速杂交到生物素化的寡聚核苷酸饵上，该寡聚核苷酸定义了目标序列 3’ 端序列。核苷酸饵-目标序列杂交体结合到链霉亲和素磁珠上，而任何 3’ 脱靶序列则通过酶学法去除。短杂交时间搭配酶学法去除 3’ 脱靶序列的方法与传统的基于杂交的富集方法相比，有着更高的测序效率。这些经过修饰的目标序列随后被转化为含有分子标签（UMI）及样本 barcode 的文库，与 Illumina 平台兼容。可用于上机测序的文库可以在一天之内制备完成。整个制备流程与大多数自动化仪器设备兼容。 NEBNext Direct HotSpot Cancer Panel 中的寡聚核苷酸饵能够捕获 50 个基因中 190 个常见癌症目标序列的双链 DNA，能够涵盖 40 kb 的序列，包括18,000 个COSMIC 数据库中所述情况。本 panel 适用于构建约 150 bp 的文库，适用于Illumina 平台 PE75 测序。 优势： 可比对到目标区域的reads 比例更高； 避免过度测序，减少每个样本的花费； 所有区域均可得到均一的测序结果，无论 GC 含量多少； 将富集和文库制备流程相结合，1 天即可完成； 能够从有限及降解的 DNA 样品中得到高质量的文库，包括 FFPE 及 ctDNA； 区分分子重复，降低假阳性突变并提高敏感度；该试剂盒的质控是独立管理的并通过功能验证。每个组分均通过标准的酶活性检测及质控，并通过严格的额外质量控制，质控情况可在每个组分的单独页面查询。
Target enrichment, coupled with next generation sequencing (NGS), enables high throughput, deep sequencing of genomic regions of interest. NEBNext Direct is a novel, hybridization-based capture method offering significant advantages over traditional in-solution hybridization and multiplex PCR protocols. In the NEBNext Direct target enrichment approach (Figure 1), fragmented DNA is rapidly hybridized to biotinylated oligonucleotide baits that define the 3´ end of each target of interest. The bait-target hybrids are bound to streptavidin beads and any 3´ off target sequence is removed enzymatically. This combination of a short hybridization time with the enzymatic removal of 3´ off target sequence enables greater sequencing efficiency relative to conventional hybridization-based enrichment methods. The trimmed targets are then converted into Illumina-compatible libraries that include unique molecular identifiers (UMI) and a sample barcode. Sequence-ready libraries are generated within one day. The procedure is compatible with most automated liquid handling instruments. The NEBNext Direct HotSpot Cancer Panel contains baits that capture both strands of DNA across 190 common cancer targets from 50 genes, encompassing approximately 40 kb of sequence and including over 18,000 COSMIC features (Table 1). The panel is designed to generate targets of roughly 150 bp, compatible with PE75 Illumina sequencing. Advantages Generate a higher percentage of your sequencing reads aligning to your targets Eliminate the need to over-sequence, reducing cost per sample Obtain uniform sequencing of all targets, regardless of GC content Save time with a 1-day workflow that combines enrichment with library preparation Generate high quality libraries with limited input amounts and degraded DNA samples, including FFPE and ctDNA Distinguish molecular duplicates, reducing false positive variants and improving sensitivity

NEBNext 超快速连接模块