二代测序文库制备,应用：制备DNA文库,Illumina平台文库制备；NEBNext Ultra II Q5? Master Mix是Q5DNA聚合酶的新配方,大大提高了文库扩增后的一致性,包括GC富集区的优化。 Master Mix的主要成分，Q5?高保真DNA聚合酶,是一种新型耐热性的DNA聚合酶,具有3?→5?核酸外切酶活性。NEBNext Ultra II Q5 Master Mix, 50 rxns

DNA模块产品,二代测序文库制备；适用于:制备DNA文库,Illumina®平台文库制备，用于新一代测序的NEBNext®试剂

二代测序文库制备,应用：制备DNA文库,Illumina平台文库制备；NEBNext Ultra II Q5? Master Mix是Q5DNA聚合酶的新配方,大大提高了文库扩增后的一致性,包括GC富集区的优化。 Master Mix的主要成分，Q5?高保真DNA聚合酶,是一种新型耐热性的DNA聚合酶,具有3?→5?核酸外切酶活性。NEBNext Ultra II Q5 Master Mix, 250 rxns

DNA模块,新一代测序文库制备产品；适用于:制备DNA库,Illumina®平台文库制备,新一代测序样品制备的NEBNext®试剂

NEB二代测序文库制备产品；适用于:制备DNA库,Illumina®平台文库制备,二代测序样品制备的NEBNext®试剂；新一代的NEBNext试剂使用快速、流线型、可自动化的工作流程,减少PCR循环次数,同提高GC含量。同时也兼容PCR-free；NEBNext Ultra II DNA Library Prep Kit for Illumina, 24 rxns

DNA模块产品,二代测序文库制备；适用于:制备DNA文库,Illumina®平台文库制备，用于新一代测序的NEBNext®试剂

DNA模块,新一代测序文库制备产品；适用于:制备DNA库,Illumina®平台文库制备,新一代测序样品制备的NEBNext®试剂

NucleoMag NGS Clean-up and Size Select 磁珠法NGS纯化及片段选择，50ml，50次

NEBNext® dsDNA Fragmentase® Reaction Buffer v2, 6.0 ml NEBNext® dsDNA片段化酶反应缓冲液，10XBuffer，1X反应体系即可确保酶的更适活性。

为新一代测序文库扩增优化的高性能超保真扩增酶预混液，超过常规Taq酶100倍的超高保真度，超强扩增能力，可用于高GC含量模板，是NGS文库构建的理想之选。Specifically optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries, regardless of GC content. The polymerase component of the master mix The NEBNext Q5 Hot Start HiFi PCR Master Mix is specifically optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries, regardless of GC content. The polymerase component of the master mix, Q5 High-Fidelity DNA Polymerase, is a novel thermostable DNA polymerase that possesses 3´→5´ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5 also has an ultra-low error rate (> 100-fold lower than that of Taq DNA Polymerase and ~12-fold lower than that of Pyrococcus furiosus (Pfu) DNA Polymerase). The buffer component of the master mix has been optimized for robust amplification, even with GC-rich amplicons and offers enhanced compatibility with a variety of beads used in typical NGS workflows. These features make the NEBNext Q5 Hot Start HiFi PCR Master Mix ideal for NGS library construction. This convenient 2X master mix contains dNTPs, Mg++ and a proprietary buffer, and requires only the addition of primers and DNA template for robust amplification. The inclusion of the hot start aptamer allows convenient room temperature reaction set up. Applications Next generation sequencing library construction High-fidelity PCR Difficult amplification High-throughput PCR

NEBNext超保真 2X PCR预混液专门针对二代测序文库制备中的PCR扩增反应进行了优化，不论GC含量多少，该产品都能够进行稳定、高保真的扩增。特殊的预混液中包含了错配率极低的 Q5 超保真 DNA 聚合酶、优化的缓冲液和反应增强剂，适合大多数模板的扩增，包括高 GC 含量的模板。该预混液的缓冲液组份针对高 GC 含量的模板甚至是二代测序文库都可提供超强的扩增。

NEBNext 超快速末端修复/加 dA 尾模块   

为新一代测序文库扩增优化的高性能超保真扩增酶预混液，超过常规Taq酶100倍的超高保真度，超强扩增能力，可用于高GC含量模板，是NGS文库构建的理想之选。Specifically optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries, regardless of GC content. The polymerase component of the master mix The NEBNext Q5 Hot Start HiFi PCR Master Mix is specifically optimized for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries, regardless of GC content. The polymerase component of the master mix, Q5 High-Fidelity DNA Polymerase, is a novel thermostable DNA polymerase that possesses 3´→5´ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5 also has an ultra-low error rate (> 100-fold lower than that of Taq DNA Polymerase and ~12-fold lower than that of Pyrococcus furiosus (Pfu) DNA Polymerase). The buffer component of the master mix has been optimized for robust amplification, even with GC-rich amplicons and offers enhanced compatibility with a variety of beads used in typical NGS workflows. These features make the NEBNext Q5 Hot Start HiFi PCR Master Mix ideal for NGS library construction. This convenient 2X master mix contains dNTPs, Mg++ and a proprietary buffer, and requires only the addition of primers and DNA template for robust amplification. The inclusion of the hot start aptamer allows convenient room temperature reaction set up. Applications Next generation sequencing library construction High-fidelity PCR Difficult amplification High-throughput PCR

NEBNext超保真 2X PCR预混液专门针对二代测序文库制备中的PCR扩增反应进行了优化，不论GC含量多少，该产品都能够进行稳定、高保真的扩增。特殊的预混液中包含了错配率极低的 Q5 超保真 DNA 聚合酶、优化的缓冲液和反应增强剂，适合大多数模板的扩增，包括高 GC 含量的模板。该预混液的缓冲液组份针对高 GC 含量的模板甚至是二代测序文库都可提供超强的扩增。

NEBNext 超快速连接模块