20 µg转染即用型，纯化的质粒DNA；更多可达20次转染；Axl CRISPR Activation Plasmid (h)是一种协同激活介质（SAM）转录激活系统，目的在于特定上调基因表达；Axl CRISPR Activation Plasmid (h)包含三种质量比为1:1:1的质粒：一种质粒编码去活化的Cas9 (dCas9)核酸酶(D10A and N863A)，它融合了转录活化域VP64，和杀稻瘟菌素抗性基因；一种质粒编码MS2-p65-HSF1融合蛋白，和潮霉素抗性基因；一种质粒编码目标特定的20 nt向导RNA，和嘌呤霉素抗性基因；形成的SAM复合物结合到转录起始位点上游大约200-250 nt的特定位点区域，为高效激活基因提供转录因子的招募；瞬时转染后，基因激活效率可用抗体通过WB, IF或者IHC测定:?Axl (B-2): sc-166268或通过WB, IF, 或IHC用抗体:Axl (C-20): sc-1096；20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections；Axl CRISPR Activation Plasmid (h) is a syne µgistic activation mediator (SAM) transcription activation system des µgned to specifically upr µgulate µgene expression；Axl CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encodi µg the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance µgene; a plasmid encodi µg the MS2-p65-HSF1 fusion protein, and a h µgromycin resistance µgene; a plasmid encodi µg a ta µget-specific 20 nt µguide RNA, and a puromycin resistance µgene；The resulti µg SAM complex binds to a site-specific r µgion approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for h µghly efficient µgene activation；Followi µg transient transfection, µgene activation efficiency can be assayed by WB, IF or IHC usi µg antibody:?Axl (B-2): sc-166268 or by WB, IF or IHC usi µg antibody:?Axl (C-20): sc-1096
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