洗涤缓冲液B(20 mL):足够做50-100个样本,次使用前须添加无核酶水。
Wash Buffer B (20 mL): Enough provided for 50-100 samples. Nuclease-free water must be added upon first use. Stellaris RNA荧光原位杂交缓冲液包含的添加剂,能够增强Stellaris RNA荧光原位杂交探针的检测信号,尤其适合于一些典型的荧光背景高的应用。
Stellaris® RNA FISH Buffers contain proprietary additives to enhance detection with Stellaris RNA FISH probe sets, particularly for applications that typically exhibit more pronounced background fluorescence.
Before getting started with your first experiments, please download our Getting Started Guide. You can also learn more about the Stellaris RNA FISH technology by downloading our white paper or by visiting our RNA FISH applications page. Furthermore, The BiosearchTech Blog contains the most up-to-date tips and tricks for running Stellaris RNA FISH experiments.
属性:
更大激发荧光(Lambda Max):495;
更大发射荧光:520;
产品用法:
溶解后的探针注意尽量避免反复冻融。当天或短期使用,探针溶液可以避光放在+2 ~ +8 °C储存一个月,超过1个月,推荐分装后将溶解后的探针放在-15至-30 °C避光保存。
Protocols:详细Protocol根据样本类型而异,请上以下网站下载:https://www.biosearchtech.com/stellarisprotocols;
储存及运输:
运输条件:常温/蓝冰/干冰;
储存条件:+2 ~ +8 °C;
Properties:
Product Usage:
Absorption Maximum (Lambda Max): 647;
Fluorescence Maximum: 670;
Product Usage:
Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month. For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month. Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols.
Storage and Handling:
Shipping Conditions: Ambient;
Storage Conditions: +2 to +8 °C 设计您自己的自定义Stellaris®RNA FISH探针来检测你感兴趣的RNA。通过使用LGC Biosearch在线Stellaris RNA FISH探针设计工具Stellaris RNA FISH probe designer,可以设计得到多达48个荧光单独标记的寡核苷酸来结合并可视化待检测的靶标RNA。在开始您的Stellaris RNA FISH自定义探针设计之前,可以通过在DesignReady探针界面的搜索框内输入感兴趣的RNA名称来确认LGC Biosearch是否已经有针对您需要检测的靶标有设计好的探针。LGC Biosearch还提供Stellaris ShipReady探针可作为您实验的阳性对照,以及Stellaris缓冲液,作为探针的完美搭配,能够让RNA的检测与分析更强大。
产品形式:一组Stellaris FISH探针集包含荧光标记的高达48种寡核苷酸的混合物,提供冻干粉形式。
交付量:5 nmol汇集的寡核苷酸(根据每种靶标更优的稀释度可以做200-400次杂交实验)。
价格:所有自定义FISH探针价格统一。
设计周期:5-7个工作日。
Design your own Custom Stellaris® RNA FISH Probe Set to detect your RNA of interest. By using our Stellaris RNA FISH probe designer, you can design up to 48 individually fluorescently labeled oligonucleotides to bind along and visualize your target RNA. Before you begin designing your Stellaris RNA FISH probe set, check to see if we have already designed a set against your target of interest by entering your target into the search box on our DesignReady page. We also offer Stellaris ShipReady probe sets to be used as controls for your experiment and Stellaris Buffers, which are the perfect accompaniment to your probe sets, allowing for even more robust RNA detection and analysis.
Product information: One set of Stellaris FISH probes contain a blend of up to 48 oligos labeled with a fluorophore. Probes arrive lyophilized and ready to use.
Delivered Amount: 5 nmol of pooled oligos (200-400 hybridization experiments depending on optimal working dilutions for each target).
Price: Same price for all custom Stellaris probes.
Turnaround time: 5 to 7 business days.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications/dinosine LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
The dI modification adds the deoxy-inosine nucleotide internally or to the 5’ end of an oligonucleotide. Inosine is often used as a degenerate base or wobble base.
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications/dinosine LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
The dI modification adds the deoxy-inosine nucleotide internally or to the 5’ end of an oligonucleotide. Inosine is often used as a degenerate base or wobble base.
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications/dinosine LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
The dI modification adds the deoxy-inosine nucleotide internally or to the 5’ end of an oligonucleotide. Inosine is often used as a degenerate base or wobble base.
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.
https://www.biosearchtech.com/support/resources/oligo-modifications LGC Biosearch提供多种多样的寡核苷酸化学修饰方式,修饰类型从碱基修饰到荧光报告基团和猝灭基团标记,修饰位置包括3'端修饰、5'端修饰以及内部修饰,可任意排列组合,种类丰比任何一家提供Oligo定制或引物合成的公司都要多。
Biosearch Technologies offers more modifications than any other custom oligo supplier. From modified bases to fluorescent reporter and quencher labels, Biosearch offers a complete array of modifications for 3', 5' and internal labeling.